how to draw blood cultures correctly

Shut off intravenous IV fluids infusing through line and clamp lumen. When drawing blood if you are collecting blood cultures and other labs make sure you collect blood cultures first before using blood for other lab tests.


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Perform hand hygiene and put on gloves.

. Steps for Drawing Blood Cultures Gather the materials needed to draw a blood culture. The first step in drawing blood correctly is to identify the appropriate veins to puncture. If collecting a blood culture that requires Isolator Microbial System AFB or fungus prep stopper with alcohol.

By using a syringe and needle to draw the patients blood you can control the pressure and speed at which the blood is removed by varying the rate that you pull back on the plunger. Use the Isolator as you would a VACUTAINER. Steps for Drawing Blood Cultures Gather the materials needed to draw a blood culture.

While wearing gloves attach syringe or Blood Culture Adapter Cap to. Insert the needle at a 15 to 30-degree angle into the vessel. Drawing blood cultures is easy once you know what to do.

Overviewthis video describes the steps needed to collect and inoculate bactalert blood culture bottles using the needle and syringe draw methodsteps inclu. Ten ml of blood is optimal in each blood culture bottle. It is acceptable but not ideal to draw blood specimens when first introducing an in-dwelling venous device before connecting the cannula to the intravenous fluids.

Using two separate alcohol preps scrub cathether hub connection for 15 seconds with 70 alcohol. Blood Cultures At least two blood cultures should be obtained with at least one drawn percutaneously and one drawn through each vascular access device unless the device was recently. Never draw blood cultures directly with a blood culture holder cap and vacutainer needle.

The materials include sterile gauze zip lock specimen bags tape patient labels self-adhesive gloves tourniquet alcohol swabs chlorhexidine swabs blood culture bottles 2 bottles for each set 2 needles 23 or 25 gauge for pediatrics and 22 gauge for adults and 2 syringes 5 cc or. Do not overfill the bottles as this can lead to. In this case nurses should take the blood collected and use it all in the aerobic bottle.

If collecting a blood culture that requires Isolator Microbial System AFB or fungus prep stopper with alcohol. A butterfly adapter should always be used when using a blood culture holder cap to prevent reflux into the patient. Always remember though that a set of blood cultures is 2 bottles at one site along with another set.

Disinfect top of blood culture bottles with an alcohol pad. Thereby the blood culture collection will serve as the discard amount. Drawing blood cultures is easy once you know what to do.


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